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For the IHC study, after deparaffinization (60 C) and antigen retrieval (1 mM citrate, pH 6, 98 C), slides were treated with 3% H 2 O 2 (5 min) and incubated with primary antibodies (1550 min): cleaved caspase-3 (1:300 dilution, Cell Signaling, 9664S), -H2AX (1:400 dilution, Abcam ab22551), HO-1 (1:400 dilution, Abcam ab52947) and CDA (1:300 dilution, Invitrogen PA5-95616) on a Leica Bond RXm autostainer

[37] Das SK, Prusty A, Samantaray D, Hasan M, Jena S, Patra JK, et al

Nigella sativa protects against isoproterenol-induced myocardial infarction by alleviating oxidative stress, biochemical alterations and histological damage

P., Iyer, P