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The raw fluorescence data were converted to hydroquinone concentrations using response factors of 4.64 10 9 RFU M 1 (for CoQcoumarin) and 3.40 10 9 RFU M 1 (for vitamin Kcoumarin) which were determined from a standard curve obtained from the maximum fluorescence recorded for various concentrations of the quinones in the presence of massive excess of either rhFSP1, FAD or NADH