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Separation was performed using an waters symmetry C18 chromatographic column (5 m*250 mm*4.6 mm), with a 25 min gradient program of 0.05 mol/L aqueous solution of CH3COONa containing 1% DMF (pH=6.5) (A) and acetonitrile (B): 16% B (1 min), 1631% B (4 min), 3136% B (9.5 min), 3655% B (11 min), 5560% B (12 min), 60100% B (15 min), 100% B (20 min), 10016% B (25 min)

Brainstem functional magnetic resonance imaging: disentangling signal from physiological noise

Previous evidence demonstrates that expression of multiple genes involved in cilia biogenesis are significantly downregulated in NHBECs when DA liquid was added to cell culture media [29]

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