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Membranes were washed with PBS and fixed with ROTIHistofix 4% for 10 min at RT

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In detail, treatment with 100 M peroxynitrite for 24 h induced minor SGN loss (75.4 2.49% SGNs survived after treatment) and the higher concentration of 200 M peroxynitrite treatment for 48 h caused approximately only 32.7 1.84% SGNs survival compared with controls