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Preservative-free options like sterile water or SWFI are generally reserved for situations where the entire reconstituted volume will be used at once, or where a particular protocol specifically calls for a preservative-free diluent

3 (download XLSX Statistical source data

Three groups were vaccinated three times at three-week intervals with the following formulations: metE-DNA (100 g/100 L PBS/mouse), MetE protein alone (1 g/100 L PBS/mouse), and 1 g metE protein combined with AddaS03 adjuvant at a 1:1 volume ratio in a total of 100 L PBS/mouse, and AS01 adjuvant (5 g monophosphoryl lipid A [MPL]/5 g QS-21 in a total of 100 L PBS/mouse, representing 1/10th of the human dose of AS01)

Initial research tested doses as low as 250mcg and as high as 1mg daily

Yes and the combination is often more effective than either alone