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doi: 10.1016/0003-2697(71)90370-8 7 CaputiL.MalnoyM.GoremykinV.NikiforovaS.MartensS

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Bound FcRn was detected either by a horseradish peroxidase (HRP)-conjugated anti-GST antibody from goat (Rockland Immunochemicals, 200-301-200) (diluted 1:5000 in PBS/T/S) or streptavidin-AP (diluted 1:5000 in PBS/T/S) and visualized by addition of TMB substrate (CalBiochem) or p-nitrophenylphosphate (AP substrate) (Sigma-Aldrich) diluted to 10 g/mL in diethanolamine buffer