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Alcohol consumption on DSIP nights is inadvisable, as alcohol already disrupts sleep architecture and combining it with DSIPs effects creates unpredictable outcomes

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The lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2 reducing Laemmli buffer and heated for ten min at 95 C