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This two-step injection protocol is known to induce OA-like cartilage degeneration, particularly in the lateral portion of the femoral condyle

This is followed by multiple immune abnormalities: marked impairment of the immunosuppressive function of myeloid-derived suppressor cells (MDSCs), pro-inflammatory polarization of Kupffer cells, T-cell differentiation imbalance, and B-cell hyperactivation that induces antimitochondrial antibody (AMA)-mediated cytotoxicity

Kim J, Hong SJ, Lim EK, Yu YS, Kim SW, Roh JH, Do IG, Joh JW, Kim DS (2009) Expression of nicotinamide N -methyltransferase in hepatocellular carcinoma is associated with poor prognosis