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Quantitative PCR (qPCR) confirmed these results (Extended Data Fig

Beissner, F., Meissner, K., Bar, K

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It plays a crucial role as a platelet activator by interacting with protease-activated platelet receptors (169)

For each biopsy, 57 representative high-power fields (20) were manually selected from tumor and normal epithelial regions using the region of interest (ROI) tool in ImageJ, while avoiding overlapping cells, necrotic zones, and staining artifacts