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Conversely, plasma EGCG levels exceeding 10 M may induce pro-oxidant activity, augmenting autophagy and cell death, and are commonly employed in tumor therapy (41)

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Matrigel tube formation assay Primary ESCs were treated with erastin (30 M) and/or NAC (10 M) in serum-free DMEM/F12, and the supernatant was collected after 12 h of incubation