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Proteolytic stability assay To evaluate the proteolytic stability of peptides against chymotrypsin, the peptides were dissolved to 1 mg/mL and incubated with chymotrypsin (1 mg/mL) at 37C for different times (0, 1, 2, 4, 8, and 16 h)

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The mean fluorescence intensity for ROS-responsive hydrogel with monomer copper peptides M-CuP (G/M-CuP) and G/D-CuP groups decreased by 71% and 63%, respectively, in comparison to the NC group (Supplementary Fig

Mechanisms of Protection: Upregulation of superoxide dismutase (SOD) and catalase key antioxidant enzymes Suppression of pro-inflammatory cytokine production (IL-6, TNF-) Activation of cellular repair pathways (autophagy, protein synthesis) Restoration of wound-healing fibroblast function Clinical Evidence & Research Applications Skin Regeneration & Photoaging Topical GHK-Cu has been investigated in multiple clinical trials examining its effects on sun-damaged skin (photoaging), wrinkles, skin thickness, and elasticity

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