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According to the latest guidelines from the American Veterinary Medical Association (50) and guidelines adopted by institutions such as Boston University (51) and the University of Maryland (52), the rats were anesthetized by intraperitoneal injection of ketamine (80 mg/kg) and xylazine (10 mg/kg), in compliance with approved ethical standards (53,54)

Transepidermal pathway The movement of a permeant across the skin occurs through both intracellular and extracellular spaces along the transepidermal pathway, starting from the epidermis and progressing to the dermis and then the hypodermis

A vesicular carrier as a novel tool for transdermal drug delivery system

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Since its discovery in the early 1990s, BPC-157 has been studied extensively in laboratory and animal models for its potential influence on cellular protection, structural repair, and angiogenic processes