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These nitrated mitochondrial proteins give rise to mitochondrial DNA damage and the opening of the mitochondrial permeability transition pore (MPTP), eventually inducing cell necrosis (Figure 2)

Glycosylation abnormalities in Gdt1p/TMEM165 deficient cells result from a defect in Golgi manganese homeostasis

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Tandem affinity purification results in more highly refined and stable complexes than single-tagged strategy alone, as this technique requires even more strict binding and elution conditions