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The plate was then spun at 2700 x g for 3 min using a plate holder centrifuge, the supernatant was discarded, and the cells were washed 1X with PBS for fixing, as described earlier, before analysis by flow cytometry (two-step), or for the chase step with an azido-tagged dye (three-step), as described earlier

These variations support their implications as experimental tools for probing ligand-specific signaling bias, receptor desensitization, and regulatory feedback mechanisms within ghrelin axis biology

Regardless of variant, ROS-mediated erythrocytic hemolysis may drive the patient into an anemic state

Boxmeer JC, et al

10.1016/j.celrep.2021.109332 28 FernandesJ.SuW.Rahat-RozenbloomS.WoleverT