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In Vitro Trapping and Screening of Reactive Metabolites Using Liquid Chromatography-Mass Spectrometry

Its like Ive been shovelling snow and the plow just drove by and now I have just removed 4 of ice from the end of the driveway and my muscles inside are strained to the max

The UHPLC mobile phase consisted of ammonium formate (solvent A) and acetonitrile:methanol 7:3, v/v (solvent B) with the following linear gradient: 25% B during 15 min, 25 to 70% B for 1 min, 70% B until 25 min, 70 to 25% B during 1 min and 25% B until 30 min