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Measurement of cellular iron Cell iron content assay kit (Solarbio, Beijing, China) was used to measure cellular iron contents based on assay contains hydroxylamine hydrochloride, which reduces Fe 3+ to Fe 2+ in cells, and under weak acid conditions, Fe 2+ and phenoline form a reddish-orange mixture, and the iron content can be calculated by measuring the OD value at 510 nm

10.3390/biomedicines8110446 116 TuH

Understanding the microbiome of diabetic foot osteomyelitis: insights from molecular and microscopic approaches

Acknowledgments UF thank the Indian Council of Medical Research-Department of Health Research (ICMR-DHR) for the award of Young Scientist

Their age ranged from 6 months to 3 years