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Reconstitution, aliquoting, and storage parameters should follow institutional SOPs and validated protocols for smallmolecule handling

After cells reached early log phase, they were harvested and resuspended in cold/hot synthetic medium with DMSO (R0K0) or with 3-IAA (R6K4)

Check with your healthcare provider if any of the following occur: Bluish-colored lips, fingernails, or palms Blurred or double vision Chest pain or discomfort Cold, clammy, pale skin Confusion Continuing ringing or buzzing, or other unexplained noise in the ears Cough Dark urine Difficulty breathing or swallowing Dizziness/lightheadedness Drowsiness Fast heartbeat Fever Headache Hearing loss Hives, itching, skin rash Irregular heartbeat or breathing Puffiness/swelling of the eyelids/eyes, face, lips, or tongue Sore throat Seizures Sweating Tremor or twitching Unusual bleeding or bruising Unusual tiredness or weakness Storage Store at controlled room temperature

All cell lines were cultured in RPMI 1640 media (Gibco) supplemented with 10% Fetal Bovine Serum (FBS) (Gibco), 1% L-Glutamine (Gibco) and 1% Penicillin/Streptomycin (Gibco)

Its part of the LimaCuscoPuno tour and offers an authentic view of Perus highlands