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Safronova VG, Matveeva NK, Avkhacheva NV, Sidelnikova VM, Vanko LV, Sukhikh GT

Sulfur amino acid restriction-induced changes in redox-sensitive proteins are associated with slow protein synthesis rates

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Total RNA was isolated from the cells using the easy-BLUE Total RNA Extraction kit (Intron Biotechnology Inc., Seongnam, Korea) and template RNA was amplified using 1 l RT reaction buffer, along with primers (Bioneer Corporation, Daejeon, Korea), dNTPs, and 0.5 U Taq DNA polymerase (Invitrogen Life Technologies, Carlsbad, CA, USA), in a final volume of 25 l

E., Kurtoglu, S., Pirgon, O