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One significant disadvantage that might prohibit CH from being utilized in medicinal formulations is its limited dissolution at neutral pH ( 2 residues on the CH polymeric chain, as when protonated at lower pH levels, it results in diminished dispersion and increased molecular aggregation (Choi et al., 2024)

Next, Luciferin Detection Reagent (100 l) was added to the wells and the plate was incubated at room temperature for 15 minutes on a plate shaker before measurement of luminescence with a plate reader

doi: 10.3390/nu12020334 134 Garca-GarcaFJMonistrol-MulaACardellachFGarrabouG

Decreased TNF- synthesis by macrophages restricts cutaneous immunosurveillance by memory CD4 + T cells during aging

Notably, the NTD-UBL fragment of NUB1 failed to stimulate ATPase activity, indicating that UBL domain binding itself has no allosteric effects