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The filter was washed two times with 100 L of UA, centrifuged at 14,000 g for 15 min, and incubated with 100 L of IAA solution (0.05 M iodoacetamide in UA) for 20 min in the dark

Therefore, we investigated the key processes associated with metastasis including migration, MMP-2 and MMP-9 (gelatinase) enzyme activity as well as the formation of micrometastases in liver tissue