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Considering that doubling times can affect cell phenotype and responses, cells were discarded after 30 population doublings from the initial culture provided by Coriell, and new cultures were started from aliquots frozen at low cell passage

Nature 408 , 994997 (2000)

Accelerated healing of Achilles tendon, MCL, and rotator cuff injuries in rat models with improved collagen fiber organization

Additional experiments also need to be performed to eliminate the possibility of contraindications of any possible adjuvants with the ASMs

The major issue with fat grafting and other biological substances is the inconsistency of experimental and clinical results, attributable to poor standardization, varied harvesting methods, and the lack of objective assessment protocols