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This is due both to the inflammatory nature of the disease itself and the therapies used to treat cancer

While this allowed larger sample sizes at earlier time points and the potential for longitudinal analysis in some animals (a fact that was ultimately not important), in the future, equalizing sample sizes may provide statistical simplicity, and allow a balanced correlation between US and IHC outcomes for a full data set

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For compounds requiring acetic acid acidification for solubility and including some hydrophobic peptides , Bacteriostatic Water may need to be acidified with sterile acetic acid to achieve the required pH for dissolution before addition to the lyophilised compound

It would be the most likely cause of your symptoms