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These high-quality reads were aligned to the arabidopsis TAIR10 genome with an average mapping efficiency of 95.0% uniquely mapped reads ( Supplementary Table 9 )

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a 156 minute gradient of up to 90% acetonitrile containing 0.1 % FA was used to elute the peptides directly into the electrospray ion source of a Q-Exactive Plus mass spectrometer (Thermo Scientific)

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