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Cells were washed once with PBS and incubated with fresh BFP media

Hu F, Xue R, Wei X, Wang Z, Luo S, Lin J, et al

Ultraviolet (UV) rays from excessive sun exposure and ionizing radiation from diagnostic tools or environmental sources can induce DNA damage directly, while also triggering ROS formation in surrounding tissues

After 30 min at 4 C, cells were washed in perm/wash buffer and resuspended with secondary Ab anti-Rabbit IgG FITC (Fisher Scientific 15303926) or PE (CST 79408 S)

That said, it may cause some unpleasant side effects, the most common of which are vomiting, especially if sorbitol is also used as an ingredient ( In very rare cases, activated charcoal has been linked to bowel blockages