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Analysis of relative gene expression data using real-time quantitative PCR and the 2(delta delta c(t)) method

The author Juarez-Maldonato A presented the highest number of collaborations (in blue, Figure 3C ), while Universidad Autonoma Agraria Antonio Narro in Mexico was the most collaborative (in red, Figure 3D )

doi: 10.3390/nu11020353 184 RuheeRTMaSSuzukiK

On the following day, cells were washed twice with PBS and serum free medium containing cGP (1100 nM, Bachem AG, Switzerland) and/or IGF-1(16.5 nM, Genentech, San Francisco, CA, USA) were added to each well to determine the efficacy of cGP on IGF-1 induced cell viability

The mice were euthanized through CO2 inhalation when the tumor volume surpassed 1500 mm or when the mice showed signs of serious weight loss, dreadful weakness, or Unhealed ulcers, indicating they were moribund