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Due to the relatively high binding affinity of ferrozine for Fe 2+ ions, many studies have used stoichiometric amounts of ferrozine, or a slight excess of chelator, to ensure that Fe 2+ ions are fully bound

Rastogi A, Bhadada SK, Bhansali A, et al

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In the citric acid cycle, Vitamin B3 (Niacin) is involved in the formation of acetyl-CoA while conversion of its co-enzyme takes places from NAD+ (nicotinamide adenine dinucleotide) to NADH (12)

This allows it to be used for multiple doses from the same vial