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Figure 8 Discussion Betaine is a common additive in aquafeeds, with diets supplemented with betaine reported to improve growth performance and feed intake in various fish species such as allogynogenetic gibel carp ( Sander lucioperca ) ( Oreochromis niloticus ) ( It has been reported that fat accumulation was associated with impairment of hepatic one-carbon metabolism ( High fat diets in fish can readily cause liver injury, with the most obvious being increased lipid deposition and higher tissue lipid contents and, in the present study, the highest hepatic lipid content was observed in fish fed the HFD diet

doi: 10.1038/s41556-019-0440-0 158 ZhangZ.YangX.SongY

Therefore, further investigation is needed to determine the specific mechanical effects of AGEs, how they interact with collagen molecules, and to identify mechanisms that may regulate AGE accumulation and removal

33 ClinicalTrials (2021a)

Progress Photos: Standardized photos (same lighting, angles, clothing) every 2-4 weeks document visual changes that measurements may miss