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For example, In view of the significant heterogeneity observed in mitochondria, it is essential to employ comprehensive methodologies, encompassing both domestic and international gene technology, multi-omics technology, single-cell sequencing, and other techniques

The reaction was stopped by the addition of 50 l of 100% trichloroacetic acid and centrifuged at 20,000 g for 5 min

This is to prevent possible desensitization to the peptide, which can occur with continuous use

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