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Detection of cell death For cell death assays, cells were washed twice in phosphate-buffered saline and then stained with Annexin V-FITC (Biovision, Mountainview, CA) and propidium iodide (PI, Sigma-Aldrich) according to the manufacturer's instructions

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Cancer-related chronic pain and its management may lead to mental disorders, and depression and anxiety in particular can alter the synthesis of inflammatory cytokines and chemokines, the metabolism of neurotransmitters and the function of the neuroendocrine system (90)