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coli DH5 and sequences were confirmed using Sanger sequencing

doi: 10.1016/0921-8734(92)90030-s, 20 HarrisA

Christopher Shade, is the founder and CEO of Quicksilver Scientific, specializes in the biological, environmental, and analytical chemistry of mercury in all its forms and their interactions with sulfur compounds, particularly glutathione and its enzyme system

3) Sonicate the solution on ice using 180 one-second bursts at high intensity with a three-second cooling period until the bacterial liquid is clear

however, this method is considered costly because a large quantity of Zn is used in this method (Zaheer et al., 2019)