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Rights reserved 2 Vol:.(1234567890) | (2024) 14:15153 | www.nature.com/scientificreports/ e second step involves adding glycine to -EC to form GSH, catalyzed by glutathione synthetase (GSS)10

[Epub ahead of print])

Overall, these findings indicate that reductions in GPx3 abundance trends with disease severity with stage 1 having the highest mean levels of GPx3 (1.99 g/ml 0.73 g/ml) and the highest K1/U1 ratio (0.86), followed by the stage 2 with a mean of 1.2 g/ml 0.54 g/ml and average K2/U2 ratio of 0.67, with the most severe form of konzo, stage 3, displaying the largest reduction in average protein abundance (mean: 1.15 g/ml 0.58 g/ml) and the lowest K3/U3 ratio of 0.59 (Supplementary Data 1)

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Log 2 -transformed relative expression values and Ct values were imported for mRNA and miRNA statistical analysis, respectively