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Firstly, water purification system is utilized to attain sterile water for injection and secondly analytical balance is used to weigh the ingredients accurately

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Marrs et al., 1984) LD 50 oral mouse: 946 mg/kg (Marrs et al., 1984) LD 50 intravenous rat: 57 mg/kg (Kiese et al., 1975) LD 50 oral rat: 689-780 mg/kg (Marrs et al., 1984) LD 50 intravenous dog: 26 mg/kg (Weger, 1975) In all five studies, the cause of death was inadequate oxygen transport by the blood to the tissues because 85% of the haemoglobin had been converted to methaemoglobin