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Flow cytometry and sorting Flow cytometric analysis was performed using an LSRFortessa cytometer (BD Biosciences), cell sorting was performed using a FACSAria II sorter (BD Biosciences), and all data were analyzed with FlowJo software (Tree Star Inc.)

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APAP will move from the intestinal tract lumen across a mucosal membrane into the bloodstream by either direct diffusion through epithelial and endothelial cells or paracellular transport [18,19]