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Room temperature storage (25C) degrades peptides 4-8 times faster than proper refrigeration (2-8C)

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Three interconnected pathways function as core suppressors of ferroptosis: (1) the GPX4-GSH system (central suppressor), (2) the SLC7A11-cystine import pathway (GSH synthesis), and (3) the AIF family member 2 (AIFM2, also known as FSP1)-NAD(P)Hquinone pathway (parallel protection) 84-87

Choosing a good binding buffer is key to making sure that youre capturing as much of your GST-fusion protein as possible