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Further development of such peptide inhibitors toward a lead therapeutic candidate, if proven to be selective for the target and relatively free of clinical side effects, may eventually lead to novel treatments for neurodegenerative disorders ranging from Alzheimers disease to Frontotemporal dementia to Parkinsons disease, says Stuart Lipton, a professor of neuroscience at Scripps Research, who was not involved in the study

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Characteristics of High Affinity Ni-Charged Resin FF 1 Dynamic binding capacity conditions: Sample: 5 mg/mL (histidine) 6 -tagged pure proteins (M r 43 000) in binding buffer (capacity at 10% Breakthrough) Column volume: 1 mL Retention time: 3min Flow rate: 0.333 mL/min Binding buffer: 20 mM sodium phosphate, 500 mM NaCl, 10 mM imidazole, pH 7.4 Elution buffer: 20 mM sodium phosphate, 500 mM NaCl, 250 mM imidazole, pH 7.4 Note: Dynamic binding capacity is protein-dependent

10.3390/ijms25073730 16 de MeloA

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