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The log values of RSL3 concentration and relative cell inhibition rates were then plotted on the x -axis and y -axis

The principle of the assays used to identify compounds that bind to the HGPRBMY14 polypeptide involves preparing a reaction mixture of the HGPRBMY14 polypeptide and the test compound under conditions and for a time sufficient to allow the two components to interact and bind, thus forming a complex which can be removed and/or detected in the reaction mixture