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Then, 10 l of 0.5 mg/ml MTT solution was added to each well, and microplates were placed in an incubator at 37 C for 4 h

Melanin is a coloration pigment produced by specialized cells called melanocytes

10.1016/j.vph.2018.02.010 76 VukojevicJ.VrdoljakB.MalekinusicD.SiroglavicM.MilavicM.KolencD.et al (2020)

JAMA Intern Med 179(5):633640

To directly observe how a concussion progresses in the brain, the researchers anesthetized mice and surgically thinned their skull bones down to about 30 micrometers thick