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To infect MEFs, 500 l of virus-containing supernatant (approximately 40% of the total) was combined with 1 ml of media and 12 g ml 1 polybrene (Santa Cruz Biotechnology, SC-134220), and then added to one well of a six-well plate containing MEFs that were plated 24 h before at around 15,600 cells per cm 2 , for a final 8 g ml 1 polybrene

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The lack of cysteine causes the potential of mitochondrial membranes to become highly polarized and lipid peroxides formation, which finally results in ferroptosis (Du et al., 4.3 ER The main site for protein production and use in the cell, the Endoplasmic Reticulum (ER), has significance for maintaining cell equilibrium because it contains a significant quantity of membrane lipids