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In another hand, in vivo experiments with transgenic mice have clarified some aspects about the topic

The final measurement at the end of this step reflects the total content of GSH (free GSH + GSSG) in the samples, to calculate the GSSG content, the free GSH content must be subtracted from the total GSH content

More research is required in this area since manipulation of the GR regulatory system is currently an intriguing target for the treatment of stress-related illnesses (168)