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Methods Cell lines and culture conditions HEK293E (ATCC, CRL-10852) and Jurkat cells (ATCC, TIB-152) were cultured in RPMI media (Gibco, #61870-010) supplemented with 10% fetal bovine serum (FBS) (Sigma, #10500-064) and 1% PenStrep (Sigma, #15140-122)

Haptoglobin At low rates of release of hemoglobin into plasma, all of the hemoglobin is attached to haptoglobin (Hp) ( 101 , 102 , 103 ), and, once irreversibly bound to haptoglobin, free hemoglobin loses its oxidizing ability ( 100 , 104 )

After three washes with PBS, the cells were treated with 0.1% NaBH 4 in PBS for 7 min at room temperature

N., Cathcart, R., Schwiers, E

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