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Additionally, the brains preference for the natural form of vitamin E is evident in the frontal cortex, hippocampus, and visual cortex, where Kuchan et al

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These samples were dried, reconstituted in 20 l of loading buffer (0.1% TFA, 1% acetonitrile), and stored at 20C for subsequent mass spectrometry (MS) analysis

Additionally, alternative elution by GST cleavage resulted in appeareance of two bands: a free X protein and GST tag