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Processing of undifferentiated 3T3-L1 pre-adipocytes (day 0) and differentiated adipocytes (day 10) were optimized for recovery and reproducibility of 1-MNA levels across cultured batches of 3T3-L1 cells (passages 7-8) and the 1-MNA levels were compared between the pre-adipocytes and adipocytes

It is important to note that B12 levels should ideally be measured before commencing replacement therapy, as supplementation rapidly normalises biochemical markers, potentially obscuring the diagnosis

Be patient and realistic about the expected results, and communicate regularly with your healthcare provider about your progress

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