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Flow cytometry was subsequently performed to demonstrate the expression of the fusion protein on the transfected CHO-S cells

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Materials Needed Before You Begin Before opening the vial, confirm you have the following on hand: | Material | Specification | |---|---| | Sterile diluent | Sterile water, 0.9% saline, or 0.5 to 1% acetic acid | | Pipettes | Calibrated micropipettes (P200 and P1000) | | Low-protein-binding tubes | 1.5 mL or 0.5 mL microcentrifuge tubes | | Filter (optional) | 0.22 micron PVDF or PES membrane | | Ice | For keeping aliquots cold during handling | | Vortex (do not use for mixing) | Keep nearby but do not vortex peptide | | Analytical balance or CoA | To confirm net peptide content per vial | | Label maker or permanent marker | For aliquot labeling | One step that researchers often overlook is confirming the net peptide content on the Certificate of Analysis (CoA) before calculating how much diluent to add

Chen, Y., Guo, W., Guo, X., Wanqing, Q