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Description

Some antibodies are not cross-linked and may come off under elution

For Co-IP in N

81 KunoT.MatsudaY.KatunumaN

Then, membranes were incubated with appropriate secondary antibodies, HRP-goat-anti-mouse (Abcam, ab6789) or HRP-goat-anti-rabbit (Abcam, ab6721), to bind the primary antibodies for 1 h at room temperature