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Erastin, a ferroptosis inducer, binds to VDAC2 and VDAC3 on the outer mitochondrial membrane, inducing mitochondrial dysfunction and oxidant release, ultimately triggering ferroptosis in tumor cells

EMP2 is a lipid raft-associated cell surface protein, and in this capacity may help orchestrate lipid metabolism relevant to the induction of ferroptosis

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Such oxidations are in direct competition with glutathione reductase and ABC transporters which usually maintain very low GSSG concentrations under physiological conditions 5,7,62,63,64

Following washes with phosphate-buffered saline with Tween (PBST), sections were incubated with appropriate secondary antibodies for 2 h at room temperature