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A typical reaction would take place at 37 C and include a reaction mixture containing d-PhgAT or HeT (0.018 mg/mL or 0.05 mg/mL respectively), sodium pyruvate (2.5 mM), (s)-methylbenzylamine (2.5 mM), PLP (0.1 mM) in potassium phosphate buffer (50 mM, pH8) and DMSO (0.25%v/v) in a final reaction volume of 1 mL 40,41

This increased calorie burn drives greater health benefits, such as improved cardiovascular fitness and disease risk reduction

Heil, M.D., FACS

Its half-life ranges from approximately 100200 min at pH 36.5, but sharply decreases to 19 min at pH 7.28.0

Spearmans correlation, r (A m ABCC1- Am GSTF2) = 0.68, r ( AmABCC2 -AmGSTF2) = 0.77, r = ( AmABCC1- AmMATE2 ) = 0.70, r = ( AmABCC2 - AmMATE2 ) = 0.77