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We recommend blocking free thiols with alkylating agents like NEM or IAM during protein extraction to stabilize the modification

Description Ipamorelin is a synthetic peptide compound that has been extensively studied in laboratory environments for its interactions with peptide signaling systems and receptor-mediated biological processes

For cell scratch assay, HaCaT cells (13 1e6) or human keratinocyte primary cultures (5 1e5) were seeded in six/twelve-well plates with Dulbeccos modified Eagles medium (DMEM) containing 5% heat-inactivated FBS or with dermal cell basal medium supplemented with keratinocyte growth kit components (obtained from ATCC, USA)

doi: 10.3390/cancers17182948 55 NovielloMManfrediFRuggieroEPeriniTOliveiraGCortesiFet al

Mallozzi, C., Di Stasi, M