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Further mechanistic studies showed that NNMTi increases intracellular NAD levels and activates the SIRT1 pathway by inhibiting NNMT, thereby suppressing the transcription of adipogenic genes and preventing the maturation of preadipocytes.This research provides a new perspective for understanding the molecular mechanism of adipocyte differentiation, confirms the key role of NNMTi in regulating adipogenesis, and lays a foundation for subsequent obesity intervention studies

The genes for inhibitors of the cell cycle (CDKN1, CDKN4D and ATM) are induced, whereas cyclins (proliferating cell nuclear antigen (PCNA), cyclin A, cyclin D1 and cyclin K) are downregulated during the period from 6 to 20 h [14]