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54 The cancer cells cause the drug resistance via providing the multiple copies of the Dihydrofolate reductase gene (could target enzyme of methotrexate)

In relationship with the pathway of negative regulation of leukocyte activation, we here found that Al-CPI upregulated the expression of cytotoxic T-lymphocyte associated protein 4 ( CTLA4 ) ( Supplementary Figure S3 ), an inhibitory receptor acting as a major negative regulator of T-cell responses