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doi:10.1016/j.plaphy.2013.05.032

For experiments, cells were cultured on round coverslips in 12-well plates and then stabilized for 30 min with Dulbecco's modified Eagle's medium supplemented with 5 mg/mL apotransferrin, 1 mg/mL bovine serum albumin, 25 mM HEPES (pH 7.4), 2 mM glutamine, 100 units/mL penicillin, and 100 mg/mL streptomycin (serum-free medium)

D-NIe-Tyr-Ile-NH- (CH 2 )5-CONH 2 was also predicted to be 42.68 percent unbound to plasma proteins in circulation, thus making it available for distribution into the tissues

7cf) as previously described